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Vampires cannot cross a thicket of wild rose or a line of salt. Vampires are compelled to stop and count every grain in a pile of grain or numerous objects (often grain) thrown into their path. More about this.
Vampires are unable to enter churches, temples, or other religious sanctuaries that represent "light" or goodness, whether they have been previously invited or not. They may only enter such holy places if they have been somehow desecrated beforehand.
The vampire, as any pagan creature, is not admitted in the holy sanctuaries of the Church but there are no testimonies that he can be harmed if he doesn’t follow the rule.
The vampire's renowned recuperative powers do have their limitations, however. A vampire cannot grow back a severed portion of its body. However, apart from the obvious periodic inconveniences, injuries like these will not critically impair a vampire's "life" or efficacy.
The only substances that cause a vampire pain and injury are weapons made of pure silver, and to a lesser degree - unless it penetrates the heart - wood.
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